DNA & RNA Isolation
DNA Isolation is the process of purifying DNA using a combination of chemical and physical methods.
DNA is not soluble in ethanol and isopropanol thus precipitation of DNA is improved by increasing the ionic strength generally using sodium acetate.
A modified and optimized method for RNA isolation was developed. It is based on a CTAB method including mercaptoethanol and PVPP with sequential Chloroform, Phenol, isoamyl alcohol extractions to remove protein, and polyhenols followed by two selective purification with LiCL and Sodium acetate in order to eliminate polysaccharedes. Though the introduced modifications are not new their addition resulted to be decisive for success in RNA isolation.
DNA & RNA Extraction
The principle of this single- step technique is that RNA is separated from DNA after extraction with acidic solution consisting chloroform,guanidinium thiocyante, phenol and sodium acetate.
The low pH sodium acetate tends to be used in some DNA extractions.
In acidic conditions, total RNA will remain in the upper aqueous phase of the entire mixture while DNA and proteins remain in the interphase or lower organic phase Recovery of RNA is then done by precipitation with isopropanol.
DNA Isolation is the process of purifying DNA using a combination of chemical and physical methods.DNA is not soluble in ethanol and isopropanol thus precipitation of DNA is improved by increasing the ionic strength generally using sodium acetate.
A modified and optimized method for RNA isolation was developed. It is based on a CTAB method including mercaptoethanol and PVPP with sequential Chloroform, Phenol, isoamyl alcohol extractions to remove protein, and polyhenols followed by two selective purification with LiCL and Sodium acetate in order to eliminate polysaccharedes. Though the introduced modifications are not new their addition resulted to be decisive for success in RNA isolation.
DNA & RNA Extraction
The low pH sodium acetate tends to be used in some DNA extractions.
In acidic conditions, total RNA will remain in the upper aqueous phase of the entire mixture while DNA and proteins remain in the interphase or lower organic phase Recovery of RNA is then done by precipitation with isopropanol.
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